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تشخیص مولکولی و PCR

تشخیص مولكولی ( Molecular Diagnosis)



آزمایش های PCR جهت بیماری های عفونی (Real Time PCR)
HBV DNA (qualitative & quantitative)
HCV RIBA & HCV RNA (qualitative & quantitative) & HCV Genotyping
HIV RNA PCR (qualitative & quantitative) & HIV Western Blot
HPV DNA PCR & HPV DNA genotyping
HDV - HAV - HSV I /II - EBV - CMV - VZV - Toxo - TB - HTLV - Brucella - H.Pylori
Mycoplasma - Chlamydia - Neisseria - Legionella

آزمایش های PCR & Sequencing جهت بیماری های سرطانی (كولوركتال -تیروئید -خون-پستان)
K- RAS Mutation by PCR NPM1 (Exon 12) by Sequencing
K- RAS (Codon 12,13) by Sequencing FLT3 –ITD Mutation by RFLP PCR
BRAF Mutation by PCR BCR – ABL / T(9:22)P190 qualification RT-PCR
K- RAS (Codon 12,13,61) by Sequencin BCR – ABL / T(9:22)P210 qualification RT-PCR
BRAF (Codon 600 and 464-469) by Sequencing BCR – ABL / T(9:22)P230 qualification RT-PCR
K- RAS (Codon 12,13,61) and BRAF (Codon 600 and 464-469) by Sequencing BCR – ABL quantification RT-PCR
Pi3k ( Exon 9 and 20) by Sequencing T(15:17)PMI-RARA for AML quantification RT-PCR
EGFR Mutation by PCR T(15:17)PMI-RARA for AML qualification RT-PCR
EGFR( Exon 19,21) by Sequencing T(8:21) AML1 – ETO for AML qualification RT-PCR
EGFR( Exon 18,19,20,21) by Sequencing T(8:21) AML1 – ETO for AML quantification RT-PCR
NRAS Mutation by PCR T(12:21) TEL – Aml for AML qualification RT-PCR
C-Kit (Exon8) by Sequencing T(1:19) E2A-PBX1 for ALL qualification RT-PCR
C-Kit (Exon9 and 11) by Sequencing T(4:11) MLL-AF4 for ALL qualification RT-PCR
C-Kit (Exon13 and 17) by Sequencing 13 q del CLL RT-PCR
PDGFR(Exon 12 and 18) by Sequencing 17 p del CLL RT-PCR
PDGFR(Exon 14) by Sequencing 11 q del ALL RT-PCR
DYP by RFLP PCR 12 Tri CLL RT-PCR
TYMS by PCR BRCA 1 Mutation by PCR
UGT1A1 by Sequencing BRCA 2 Mutation by PCR
PTEN ( Exon 7,8,9 and 10) by Sequencing Oncotype 21 gene by RT-PCR
JAK2-V617F Mutation by PCR C-Myc by Amplified qPCR
JAK2 (Exon 12) by Sequencing HER 2 Mutation by Amplified qPCR
MPL( Exone10) by Sequencing MPL – W515K and W515L Mutation by PCR
NPM1 Mutation by PCR
Thrombophilia Pannel
Factor V(G1691A)- Leiden PCR Factor V(H1299R)- R2
Prothrombin G20210A Mutation by PCR Factor XIII (V34L) Mutation by PCR
B Fibrinogen (- 455G>A) PAI- 1 (4G/5G)
HPA1(a/b) MTHFR(C677T) Mutation
MTHFR(A1298C) Mutation ACE(I/D)
APOB( R3500Q) APOE – Codon 112
APOE- Codon 158